Application

Western Blotting Analysis: A representative lot detected wild-type and the V263G mutant form of recombinant human aprataxin (Kijas, A.W., et al. (2006). J. Biol. Chem. 281(20):13939-13948).
Western Blotting Analysis: A representative lot detected aprataxin in lysates from HeLa and control lymphoblastoid cell lines (C3ABR and C2ABR), but not from AOA1 cell lines (L939, L938) derived from patients with the neurological disorder ataxia with oculomotor apraxia type 1 (AOA1) (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nucleolar proteins nucleolin and nucleophosmin with aprataxin from C3ABR lymphoblastoid cell lysate (Becherel, O.J., et al. (2006). Hum. Mol. Genet. 15(14):2239-2249).

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & Repair

Anti-Aprataxin Antibody is an antibody against Aprataxin for use in Western Blotting, Immunoprecipitation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Aprataxin (UniProt Q7Z2E3; also known as FHA-HIT, Forkhead-associated domain histidine triad-like protein) is encoded by the APTX (also known as AOA, AOA1, AXA1, EAOH, EOAHA) gene (Gene ID 54840) in human. Aprataxin contains an N-terminal forkhead-associated (FHA; a.a. 38-87) domain akin to that found in the DNA end damage repair factor polynucleotide kinase phosphatase (PNKP), followed by a histidine triad (HIT; a.a. 182-287) domain found in nucleoside hydrolases and transferases, and a C-terminal C2H2 type Zinc finger domain. Aprataxin exhibits both DNA- and RNA-binding activity and plays a role in reversing toxic 5′-adenylated (5′-AMP) DNA lesions generated by DNA ligases when encountering nicked DNA structures with abnormal DNA termini. Aprataxin functions as a DNA ligase proofreader to directly reverse damaged 5′-adenylated termini of DNA strand breaks that have been subjected to DNA damage-induced abortive processing by DNA ligases. Ataxia with oculomotor apraxia type 1 (AOA1) is an autosomal recessive neurological syndrome caused by an impairment in DNA repair as a result of APTX gene mutations. The related type 2 disorder AOA2 is caused by a Senataxin-deficiency due to SETX gene mutations.

Immunogen

Recombinant full-length human aprataxin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antibody in serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aprataxin in 10 µg of HeLa cell lysate.

Specificity

Expected to react with all 13 human aprataxin spliced isoforms reported by UniProt (Q7Z2E3-1 through Q7Z2E3-13).

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~43 kDa observed.