Analysis Note

ControlHeLa cell lysate

Application

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected HEXIM1 in HeLa and A431 cells.

Use Anti-HEXIM1 Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect HEXIM1 also known as Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein ,Hexamethylene bis-acetamide-inducible protein 1.

Research Sub CategoryTranscription FactorsChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HEXIM1 is a transcriptional regulator that functions as a general RNA polymerase II transcription inhibitor. HEXIM1 works in cooperation with 7SK snRNA to sequester P-TEFb in a large inactive 7SK snRNP complex, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK snRNP complex is composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Hexim1 also interacts with the N-CoR complex through NCOR1, ESR1, NR3C1, and may interact with NF-kappa-B through REL A. HEXIM1 is located in the nucleus where it can bind with alpha-importin, and can also be found in the cytoplasm. It is up-regulated by HMBA (hexamethylene bisacetamide) at the protein level and down-regulated by estrogen.

Immunogen

Recombinant protein corresponding to human HEXIM1.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected HEXIM1 on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~52 kDa observed. An uncharacterized band at ~60 kDa has been observed in some lysates.