Application

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected phospho Mis18-binding protein 1 (Thr702) in HeLa cells.
Dot Blot Analysis: 1 µg/mL from a representative lot detected the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis.
Immunocytochemistry Analysis: A representative lot detected Thr702 phosphorylated Mis18-binding protein 1/M18BP1 localized at the centromeres in HeLa cells (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411).
Western Blotting Analysis: A representative lot detected in vitro phosphorylation of recombinant Mis18-binding protein 1/M18BP1 on Thr702 by recombinant polo-like kinase 1 (Plk1) (McKinley, K.L., and Cheeseman, I.M. (2014). Cell. 158(2):397-411).

Research CategoryEpigenetics & Nuclear Function

Anti-phospho Mis18-binding protein 1 (Thr702) Antibody is an antibody against phospho Mis18-binding protein 1 for use in Immunocytochemistry, Dot Blot, Western Blotting.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mis18-binding protein 1 (UniProt Q6P0N0; also known as hsKNL-2, Kinetochore null 2 homolog, Kinetochore-associated protein KNL-2 homolog, P243, Putative protein p243 which interacts with transcription factor Sp1) is encoded by the MIS18BP1 (also known as C14orf106, KIAA1903, KNL2, M18BP1) gene (Gene ID 55320) in human. Mis18-binding protein 1 is a component of the Mis18 complex (Mis18α, Mis18β, and M18BP1/KNL2) that plays a role in mediating cell cycle-dependent deposition/incorporation of the histone H3 variant CENP-A at the centromeres. CENP-A incorporation is carried out by at least two sets of events, the Mis18 complex must first localize to centromeres at the onset of anaphase, HJURP-mediated CENP-A deposition then occurs during G1. Polo-like kinase 1 (Plk1) is shown to phosphorylate M18BP1 on Thr702 and plays an important role in promoting Mis18 complex centromere localization.

Immunogen

Linear peptide corresponding to a human M18BP1 sequence containing phosphorylated Thr702.

Epitope: M18BP1 pThr702

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.

Quality

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected phospho Mis18-binding protein 1 (Thr702) in A431 cells.

Specificity

Specifically reacts with the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis. Phosphorylation site is not present in the spliced isoform 2 of human Mis18-binding protein 1/M18BP1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

129 kDa calculated.