Application
Western Blotting Analysis: 2 µg/mL from a representative lot detected Adenosylhomocysteinase (AHCY) in 200 µg of human placental tissue lysate.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Adenosylhomocysteinase (AHCY) in human kidney and human skin tissues.
Anti-Adenosylhomocysteinase (AHCY) Antibody, Cat. No. ABE1803, is a highly specific rabbit polyclonal antibody that targets Adenosylhomocysteinase (AHCY) and has been tested for use in Immunohistochemistry (Paraffin), and Western Blotting.
Research CategoryEpigenetics & Nuclear Function
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Adenosylhomocysteinase (UniProt: P23526; also known as EC: 3.3.1.1, AdoHcyase, S-adenosyl-L-homocysteine hydrolase) is encoded by the AHCY (also known as SAHH) gene (Gene ID: 191) in human. Adenosylhomocysteinase is a homotetrametic enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine. Hence, it regulates the intracellular S-adenosylhomocysteine concentration, which is thought to be important for transmethylation reactions. Two isoforms of adenosylhomocysteinase have been reported that are produced by alternative splicing. It contains three nucleotide binding regions (aa 157-159; 222-227; and 299-301). Defects in AHCY gene cause hypermethioninemia that is characterized with failure to thrive, mental and motor retardation, facial dysmorphism with abnormal hair and teeth, and myocardiopathy.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminus region of human Adenosylhomocysteinase.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4, with 0.05% sodium azide.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Adenosylhomocysteinase (AHCY) in 200 µg of HeLa cell lysate.
Specificity
This rabbit polyclonal antibody detects Adenosylhomocysteinase in human. It targets and epitope within 12 amino acids from the C-terminus region.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Target description
~47 kDa observed; 47.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
This product has met the following criteria: