Application

Anti-SMUG1, Cat. No. ABE1798, is a highly specific rabbit polyclonal antibody that targets single-strand selective monofunctional uracil DNA glycosylase and has been tested in Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Single-strand selective monofunctional uracil DNA glycosylase (UniProt: Q53HV7; also known as SMUG1, hSMUG1) is encoded by the SMUG1 gene (Gene ID: 23583) in human. SMUG1 is a base excision repair enzyme that removes uracil and oxidized pyrimidines from DNA. It recognizes base lesions in the genome and initiates base excision DNA repair. It displays greater activity toward mismatches (U/G) compared to matches (U/A). SMUG1 excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. Human and rat SMUG1 exhibit about 84% sequence homology and mouse SMUG1 has 94% homology with rat SMUG1. SMUG1 was originally identified as a monofunctional uracil-DNA glycosylase selective for ssDNA, however, later it was shown to be active for U in dsDNA. SMUG1 displays the following substrate preference: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration; and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration. SMUG1 preferentially binds to a dsDNA substrate over an ssDNA substrate when both are present. SMUG1 is shown to be a pseudouridine synthase dyskerin (DKC1) partner that contributes to rRNA quality control, partly by regulating 5-hydroxymethyluridine levels. (Ref: Matsubara, M et al. (2003). Biochemistry 42(17): 4993-5002; Masaoka A., et al. (2003). Biochemistry. 42(17): 5003-5012; Jobert, L et al. (2013). Mol. Cell 49(2): 339-345).

Immunogen

KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human SMUG1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected SMUG1 in 10 µg of HeLa cell nuclear extract.

Specificity

This polyclonal antibody detects SMUG1 in HeLa cell nuclear extract. It targets an epitope within 13 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~34 kDa observed; 29.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).