Application

Research CategoryEpigenetics & Nuclear Function

Anti-PAXX, Cat. No. ABE1795, is a highly specific rabbit polyclonal antibody that targets PAXX (C9orf142) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PAXX in human prostate, human tonsil, and human cervical cancer tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein PAXX (UniProt: 9BUH6; also known as Paralog of XRCC4 and XLF, XRCC4-like small protein) is encoded by the C9orf142 (also known as PAXX, XLS) gene (Gene ID: 286257) in human. PAXX is a newer member of XRCC4 superfamily that predominantly localizes to the nucleus and is shown to accumulate at sites of DNA damage generated by laser microirradiation. It is involved in non-homologous end joining (NHEJ), a major pathway to repair double-strand breaks in DNA and may act as a scaffold required to stabilize the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites where it promote the assembly and/or stability of the NHEJ machinery. PAXX residues 1-113 form a head domain, which is structurally related to those of XRCC4, XLF and SAS6. This is followed by a 31-amino-acid -helix, forming a coiled-coil with the other protomer to make a PAXX homodimer. Two isoforms of PAXX have been described that are produced by alternative splicing. (Ref.: Ochi, T., et al. (2015). Science 347(6218); 185-188).

Immunogen

KLH-conjugated liner peptide corresponding to 16 amino acids from the C-terminus region of human Protein PAXX.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected PAXX in HeLa cell nuclear extract.

Specificity

This rabbit polyclonal antibody detects PAXX protein. It targets an epitope within 16 amino acids from the C-terminus region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed; 21.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).