Application

Research CategoryEpigenetics & Nuclear Function

Anti-Nicotinamide N-methyltransferase Antibody, Cat. No. ABE1788, is a highly specific rabbit polyclonal antibody that targets human Nicotinamide N-methyltransferase and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Western Blotting Analysis: 1 µg/mL from a representative lot detected Nicotinamide N-methyltransferase in 10 µg of human kidney lysate and HEK293 cell lysate.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nicotinamide N-methyltransferase in human liver, human kidney, and human pancreas tissues.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nicotinamide N-methyltransferase (UniProt: P40261; also known as EC: 2.1.1.1) is encoded by the NNMT gene (Gene ID: 4837) in human. Nicotinamide N-methyltransferase is a monomeric, cytoplasmic enzyme that belong to the class I -like SAM-binding methyltransferase superfamily. It catalyzes the N-methylation of nicotinamide and other pyridines with S-Adenosyl-L-methionine (SAM)) serving as the methyl donor. It has two SAM binding sites (aa 63-64 and 142-143). Its catalytic activity is important for biotransformation of many drugs and xenobiotic compounds. Nicotinamide N-methyltransferase is predominantly expressed in the liver. A lower level of expression is seen in the kidney, lung, skeletal muscle, placenta, and heart and it is not detected in the brain or pancreas. Nicotinamide N-methyltransferase inhibition is shown to increases adipose SAM and NAD(+) levels and upregulate ornithine decarboxylase and spermidine-spermine N(1)-acetyltransferase activity and expression. (Ref.: Kraus, D et al. (2014). Nature. 508 (7495); 258-62).

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Nicotinamide N-methyltransferase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nicotinamide N-methyltransferase in 10 µg of HepG2 cell lysate.

Specificity

This rabbit polyclonal antibody detects human Nicotinamide N-methyltransferase. It targets an epitope within 15 amino acids from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~30 kDa observed; 29.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).