Application

Anti-phospho-DNA-dependent RNA Pol II, subunit B1 (Ser2/5) | ABE1471 is an antibody against phospho-DNA-dependent RNA Pol II for use in Western Blotting.

General description

DNA-directed RNA polymerase II subunit RPB1, also known as RNA polymerase II subunit 1, RNA polymerase II subunit B1, DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B220, and encoded by the gene name RPO21, RPB1, RPB220, SUA8, is a member of the RNA polymerase beta chain family. DNA-dependendent RNA Pol II has been shown to catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. DNA-dependendent RNA Pol II is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. During a transcription cycle, Pol II, general transcription factors and the Mediator complex assemble as the preinitiation complex (PIC) at the promoter. Pol II is composed of mobile elements that move relative to each other. The core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center. The clamp element (portions of RPB1, RPB2 and RPB3) is connected to the core through a set of flexible switches and moves to open and close the cleft. In elongating Pol II, the lid loop (RPB1) appears to act as a wedge to drive apart the DNA and RNA strands at the upstream end of the transcription bubble and guide the RNA strand toward the RNA exit groove located near the base of the largely unstructured CTD domain of RPB1. The rudder loop (RPB1) interacts with single-stranded DNA after separation from the RNA strand, likely preventing re-association with the exiting RNA. The cleft is surrounded by jaws: an upper jaw formed by portions of RBP1, RPB2 and RPB9, and a lower jaw, formed by RPB5 and portions of RBP1. The jaws are thought to grab the incoming DNA template, mainly by RPB5 direct contacts to DNA.

Immunogen

KLH-conjugated linear peptide corresponding to human phospho-DNA-dependent RNA Pol II, subunit B1 (Ser2/5) near the C-Terminus.

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-DNA-dependent RNA Pol II, subunit B1 (Ser2/5) in 10 µg of NIH/3T3 cell lysate.

Target description

~220 kDa observed