Application

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot was used with HeLa acid extract for peptide block analysis.



Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides were probed with Anti-monomethyl Histone H2B (Pro1) (20 ng (A), 10 ng (B), 5 ng (C), 2.5 ng (D) dilutions).



Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected monomethyl Histone H2B (Pro1) in HeLa, A431, HUVEC, and NIH3T3 cell lines.

Research CategoryEpigenetics & Nuclear Function

Anti-monomethyl Histone H2B (Pro1) Antibody, Cat. No. ABE1464, is a highly specific rabbit polyclonal antibody that targets Histone H2B monomethylated on proline 1 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H2B type 1-C/E/F/G/I (UniProt: P62807; also known as Histone H2B.1 A, Histone H2B.a, H2B/a, Histone H2B.g, H2B/g, Histone H2B.h, H2B/h, Histone H2B.k, H2B/k, Histone H2B.l, H2B/l) is encoded by the HIST1H2BC (also known as H2BFL, HIST1H2BE, H2BFH, HIST1H2BF, H2BFG, HIST1H2BG, H2BFA, HIST1H2BI, H2BFK) gene (Gene ID: 3017, 9339, 8343, 8344, 8346, 8347) in human. Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability; and consequently, transcription, and DNA replication and repair. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries, which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail, H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone H2B can be monoubiquitinated on Lysine123 by RAD6/UBC2-BRE1 complex to form H2BK123Ub1 complex that gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. H2BK123ub1 is reported to modulates the formation of double-strand breaks during meiosis and is a required for DNA-damage checkpoint activation. Methylation of N-terminus Proline 1 of histone H2B (Pme) by NTMT (CG1675) is one of the rare modification that has been addressed. Heat shock or arsenite treatment is shown to increase methylation on proline 1 in Drosophila cells. (Ref.: Villar-Garea, A., et al. (2012). Nucl. Acid Res. 40(4); 1536 1549; Desrosiers, R., and Tanguay, RM. (1998). J. Biol. Chem. 263(10):4686-4692).

Immunogen

KLH-conjugated linear peptide corresponding to the first 9 amino acids (methiionine removed) from the N-terminus region of human Histone H2B1C with methylated proline.

Epitope: N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cells acid extract.

Western Blotting Analysis: 12 µg/mL of this antibody detected monomethyl Histone H2B (Pro1) in HeLa acid extract

Specificity

This rabbit polyclonal antibody specifically detect Histone H2B monomethylated on proline 1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed; 13.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).