Application

Anti-NR4A1 (Nur77), Cat. No. ABE1454, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 1 and has been tested for use in Immunocytochemistry and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected NR4A1 (Nur77) in 10 µg of COS7 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NR4A1 (Nur77) in HeLa, A431, HUVEC, and NIH/3T3 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear receptor subfamily 4 group A member 1 (UniProt: P22736; also known as Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3) is encoded by the NR4A1 (also known as GFRP1, HMR, NAK1) gene (Gene ID: 3164) in human. Nur77 is an orphan nuclear receptor that may act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. It is shown to be expressed in fetal muscle and in adult liver, brain, and thyroid and is reported to be induced by growth stimulating agents. It binds the NGFI-B response element (NBRE) 5′-AAAAGGTCA-3′. Its DNA binding region is localized to amino acids 264-339. It contains two zinc finger domains (aa 267-287 and 303-327). Nur77 is shown to heterodimerize with retinoid X receptor alpha and is actively transported from the nucleus to the mitochondria in cells undergoing apoptosis. It binds to Bcl-2 and triggers a conformational change that exposes the pro-apoptotic BH3 domain. This converts Bcl-2 from an anti-apoptotic to a pro-apoptotic factor. In several cancers the nuclear-mitochondrial translocation of Nur77/TR3 is defective, which prevents apoptosis. Three isoforms of Nur77 have been described that are produced by alternative splicing. It is acetylated by p300/CBP that increases its stability. It can also undergo phosphorylation at Serine 351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Nuclear receptor subfamily 4 group A member 1 (NR4A1). The immunogen sequence is conserved in all 3 isoforms.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NR4A1 (Nur77) in 10 µg of HeLa cell lysate.

Specificity

This rabbit polyclonal antibody detects Nuclear receptor subfamily 4 group A member 1 (NR4A1) in human, Monkey, and Murine cells It targets an epitope within 17 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~64 kDa observed; 64.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).