Application

Western Blotting Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in whole cell neuronal lysate (Courtesy of Erica Korb, Ph.D., Rockefeller University).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Brd4 (Ser492/Ser494) in cultured cortical cells from mouse (C47blac6). (Courtesy of Erica Korb, Ph.D., Rockefeller University).

Dot Blot Analysis: A representative lot detected phospho Brd4 (Ser492/Ser494) in phospho Brd4 peptides (Courtesy of Erica Korb, Ph.D., Rockefeller University).

Anti-phospho Brd4 (Ser492/Ser494), Cat. No. ABE1453, is a highly specific rabbit polyclonal antibody that targets Bromodomain-containing protein 4 and has been tested in Dot Blot, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Bromodomain-containing protein 4 (UniProt O60885; also known as MCAP, Mitotic chromosome-associated protein, Protein HUNK1) is encoded by the BRD4 (also known as HUNK1, MCAP) gene (Gene ID 23476) in human. Bromodomain-containing protein 4 is a BET (bromodomain and extra-terminal) protein family member that functions as a chromatin reader by binding acetylated lysines in histones. It plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. It remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for post-mitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure. During interphase, it plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Bromodomain-containing protein 4 is required to form the transcriptionally active P-TEFb complex where it displaces negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb and transforms it into an active form that can then phosphorylate the C-terminal domain of RNA polymerase II. It promotes the phosphorylation of Ser2 of the C-terminal domain of RNA polymerase II. In addition to acetylated histones, Bromodomain-containing protein 4 also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B. It is also reported to act as a regulator of p53/TP53-mediated transcription and following phosphorylation by casein kinase 2K2, it is recruited to p53/TP53 specific target promoters. BRD4 knockout in mice is shown to be lethal, while BRD4 function blockage by inhibitor JQ1 (Cat. No. 500586) treatment is reported to affect the transcription of critical synaptic proteins, resulting in memory deficits and decreased seizure susceptibility in mice.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids surrounding Ser492/Ser494 from the internal N-terminal half of human phospho Brd4 protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in humans IPS cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected phospho Brd44 (Ser492/Ser494) in 10 µg of human IPS cell lysate.

Specificity

This rabbit polyclonal antibody detects Bromodomain-conntaining protein 4 phosphorylated at Ser492/Ser494. It targets an epitope of 14 amino acids in the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

200 kDa observed, 152.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).