Analysis Note

ControlA431 cell lysate

Application

Anti-Atg4D Antibody is an antibody against Atg4D for use in WB, IH(P).

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Immunohistochemistry (Paraffin) Analysis: A 1:200 dilution of a previous lot detected Atg4D in human testicular cancer tissue.

Western Blot Analysis: 1 µg/mL from a representative lot detected Atg4D on 10 µg of human fetal skeletal muscle tissue, NIH/3T3, and L6 cell lysates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Atg4D (autophagy-related protein 4D) is a member of the Atg4 family of C54 endopeptidases that are required for the synthesis of autophagosomes. Atg4D is found to be cleaved by caspase-3 into a more active form where it can interact with the autophagosome marker Atg8. Atg4D is also known to be associated with mitochondrial dysfunction and the induction of apoptosis.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human Atg4D.

Epitope: N-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected Atg4D in 10 µg of A431 cell lysate.

Specificity

This antibody recognizes Atg4D at the N-terminus.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~56 kDa observed.
The calculated molecular weight is 53 kDa; however, Atg4D has been shown as a ~55 kDa band in western blots (Li, M., et al. (2011). J Biol Chem. 286(9):7327-7338.)