Analysis Note

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Application

Streptavidin fromStreptomyces avidinii has been used:in the pre-functionalization of self-propelled catalytic micromotorsin the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substratesas an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Biochem/physiol Actions

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

General description

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Other Notes

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Packaging

5, 10 mg in glass bottle

The sales quantitites 1 mg and 5 mg

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of protein which binds 1µg (+)-biotin at pH 7.5