Application
Spermidine trihydrochloride is used for the following applications: DNA extraction and restriction endonuclease digestionNuclear transplantationDuring the chemical analyses of the squid species Todaropsis eblanaeAs one of the agents in intercalating drug.Platelet aggregationIt may be used in the synthesis of palladium complexes like [sperH3]2[PdCl4]3, [PdCl2(sperH)]2[PdCl4], and [(PdCl2)3(sper)2] by reacting with K2PdCl4 and PdCl2.
Biochem/physiol Actions
Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.
Caution
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.
General description
Spermidine trihydrochloride is an aliphatic polyamine. Its crystalline structure has been studied. It has been reported to preserve the nuclei and prevent proteolytic degradation of histones during the macronuclei isolation from Tetrahymena pyriformis.
Other Notes
Buffer component for chromosome isolation in bivariate flow cytogenetic analysis and sorting.
This product has met the following criteria: