Analysis Note

Positive ControlVanadate-treated A431 cells

Application

ELISA (0.05 µg/ml)r>Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)r>Immunocytochemistry (1-10 µg/ml)r>Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)

General description

This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).

Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.

Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158.Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550.Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536.Harbeck, B., et al. 2000. J. Biol. Chem.275, 40.Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from PBS, PEG, sucrose.

Reconstitution

Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)