Biochem/physiol Actions
Cell permeable: no
Reversible: no
Product does not compete with ATP.
Primary TargetSpecifically competes against VEGF165 binding to recombinant NRP-1
Target IC50: 60 µM for specifically competes against VEGF165 binding to recombinant NRP-1 in cell-free binding assays
General description
An NRP-1-binding heptapeptide whose target was initially mistaken as VEGFR2/KDR/Flk-1 due to its binding activity to a CHO-KDR culture that was later shown to express primarily NRP-1 and little KDR. V1/A7R specifically competes against VEGF165 binding to recombinant NRP-1 in cell-free binding assays (IC50 = 60 µM), while exhibiting no activity against the interaction between NRP-1 and heparin or VEGF165 binding to KDR, VEGFR1/Flt-1, and heparin. V1/A7R is demonstrated to inhibit VEGF-dependent angiogenesis activity in HMVEC (IC50 = 0.3 µM) and HUVEC cultures in vitro, as well as in a rabbit corneal neovascularization model and in mice bearing MDA-MB-231-derived tumor in vivo.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Starzec, A., et al. 2006. Life Sci.79, 2370.Binetruy-Tournaire, R., et al. 2000. EMBO J.19, 1525.
Packaging
1 mg in Plastic ampoule
Packaged under inert gas
Sequence
H-Ala-Thr-Trp-Leu-Pro-Pro-Arg-OH
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria: