Biochem/physiol Actions
Cell permeable: no
Reversible: no
Product does not compete with ATP.
Primary TargetCalcium Probes
General description
Binding of Ca2+ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.
Binding of Ca2+ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.Bryant, D.T. 1985. Biochem. J.226, 613.Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.Ashley R.H., et al. 1984. Biochem. J.219, 149.Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.Tsien, R.Y. 1980. Biochemistry19, 2396.
Packaging
50 mg in Plastic ampoule
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.
Warning
Toxicity: Irritant (B)
This product has met the following criteria: