Biochem/physiol Actions

Cell permeable: no

Reversible: no

Product does not compete with ATP.

Primary TargetCalcium Probes

General description

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.Bryant, D.T. 1985. Biochem. J.226, 613.Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.Ashley R.H., et al. 1984. Biochem. J.219, 149.Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.Tsien, R.Y. 1980. Biochemistry19, 2396.

Packaging

50 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Warning

Toxicity: Irritant (B)