General description

pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.

Native L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.

Native L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD⁺-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.Schütte, H., et al. 1985. Appl. Microbiol.Biotechnol.22, 306.

Packaging

100 u in Plastic ampoule

Physical form

In 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Unit Definition

One unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD⁺ per min at 30°C, pH 10.7.

Warning

Toxicity: Standard Handling (A)