Analysis Note
enzyme activity: the optimum temperature is 95-100 °C (the enzyme is stable up to 75 °C), the optimum pH is 6-9 (stable from pH 6-9). Inhibitors: PCMB, Hg+.
Other Notes
An enzyme that removes pyroglutamic acid (pGlu) from pGlu-peptide and proteins; employed in Edman degradation.
Packaging
package of 0.01 Unit
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1μmol Pyroglutamate-p-nitroanilide per minute at pH7.0 and 37°C
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