Application

Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures. Because of its high collagenase activity Collagenase P is particularly suitable for the isolation of pancreatic islets from mouse and rat (function tested). The preparation is also suitable for the isolation of adipocytes from epididymal fat pads of rats.

Features and Benefits

Contents: Lyophilizate, nonsterile

General description

Collagenase P is an enzyme mixture prepared from the extracellular culture filtrate of a special Clostridium histolyticum strain.Collagenase P is a research biochemical, not manufactured under the US good manufacturing practices (GMP) regulations.Note: Collagenase P is a non-sterile preparation of the culture supernatant of Clostridium histolyticum. It is neither designed nor intended for isolation of pancreatic islets for transplantation into humans and must not be used for such purposes.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: Ca²⁺Inhibitors:Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanolCollagenase is not inhibited by serum.Clostripain inhibitors: TLCKTrypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)Working concentration: 0.5 to 2.5mg/ml0.5 to 1.5mg/ml for the isolation of pancreatic islets from mouse and rat pancreas.Approximately 2mg/ml for the isolation of adipocytes.Storage conditions (working solution): -15 to -25°CThe reconstituted solution is stable at -15 to -25°C. Prepare appropriate aliquots and avoid repeated freezing and thawing.

Reconstitution

Reconstitution in any balanced salt solution (e.g., HBSS)

Specificity

Enzyme activity:Collagenase activity: >1.5U/mg (according to Wünsch) (+25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activityCollagenase P has a very high collagenase activity (>1.5U/mg), and is function tested for the isolation of mouse pancreatic islets.Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.