Application

DNA Molecular Weight Marker for agarose gels; simplifies accurate molecular weight determination of double-stranded DNA fragments generated by: Restriction digests PCR RT-PCR

General description

Fragment mixture prepared by cleavage of SPP1-DNA with restriction endonuclease EcoR I.The product is also available as DNA Molecular Weight Marker VII, Digoxigenin-labeled.Size Range: 0.081 to 8.57 kbp

ContentsReady-to-use solution, 250 µg/ml, in TE buffer (Tris-HCl, 1 mM EDTA, pH 8.0).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Storage conditions (working solution): 2 to 8 °COnce thawed store at 2 to 8 °C.

Sequence

The mixture contains seventeen fragments with the following base pair lengths (1 base pair = 660 daltons): 81, 359, 492, 710, 718*, 992, 1164, 1482, 1515, 1882, 1953, 2799, 3639, 4899, 6106, 7427, and 8576 as determined by computer analysis of the SSP1 sequence.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.*Alonso, J.C. and Trautner, T.A., personal communication of unpublished results, the 718-bp fragment is present in non-stoichometric amounts.

Unit Definition

50 µg = 1A₂₆₀ unit