Application

Use Anti-Digoxigenin-Rhodamine, Fab fragments for the detection of digoxigenin-labeled compounds using: Digoxigenin-labeled sugars in glycoconjugate researchFluorescent in situ hybridization (FISH)ImmunohistocytochemistryIn situ hybridizationDNA fiber assay

General description

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to rhodamine.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: Detection of digoxigenin-labeled sugars in glycoconjugates: 20 to 50 μg/ml•Fluorescent in situ hybridization (FISH): 1 to 20 μg/ml•Immunohistocytochemistry: 20 to 50 μg/ml•In situ hybridization: 20 to 50 μg/mlWorking solution: PBS, 0.5% bovine serum albumin (w/v), pH 7.4.1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore pH can be increased up to pH 8.5 to 9.0.Storage conditions (working solution): Always prepare fresh!

Reconstitution

Add 1 ml double-distilled water to a final concentration of 200 µg/ml.

Specificity

Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).