Analysis Note

Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl₂ in the range of 10^-4 to 10^-3 M.

Application

Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.

Biochem/physiol Actions

Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.

General description

Acylase I belongs to the aminoacylase family of enzymes.

Other Notes

Resolution of acetyl amino acids

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 µmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C