Application

Acriflavine is used to fluorescently label high molecular weight RNA molecules .Excitation and emission wavelengths in various solvents : Methanol: λex = 424 nm; λem = 518 nm Ethanol: λex = 426 nm; λem = 524 nm Propanol: λex = 430 nm; λem = 512 nm Butanol: λex = 430 nm; λem = 526 nm Formamide: λex = 434 nm; λem = 524 nm Glycerol: λex = 432 nm; λem = 540 nm Water: λex = 416 nm; λem = 514 nmInsoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .

Other Notes

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously