Components
Methylene blue 1 g/L, ethanol (95 %) 50 mL/L, glacial acetic acid 50 mL/L in water
General description
Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.
This product has met the following criteria: