Analysis Note

ControlHuman liver tissue lysate.

Application

Research Sub CategoryHistones

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PRDM12 in NIH/3T3 and A431 cells.

Use Anti-PRDM12 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PRDM12 also known as PR domain zinc finger protein 12, arginine methyltransferase.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger gene RIZ1 is a tumor suppressor gene and the founding member of the PR domain family. PRDM12 (PR domain zinc finger protein 12) also acts as a tumor suppressor and is linked to the pathogenesis of chronic myeloid leukemia. PRDM12 is also thought to play a role in transcriptional regulation.

Immunogen

KLH-conjugated linear peptide corresponding to human PRDM12.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 3 µg/mL of this antibody detected PRDM12 on 10 µg of human liver tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed