Analysis Note

ControlMouse brain nuclear extract

Application

Detect the KIF5A protein using this Anti-KIF5A validated for use in WB & ICC.

Research CategoryNeuroscience

Immunocytochemistry Analysis: 5 µg/mL from a representative lot detected KIF5A in K562 cells.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Kinesin heavy chain isoform 5A (KIF5) is a member of the kinesin-like protein family. These proteins are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5A performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5A was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. Mutations in one of the isoforms of KIF5 (KIF5A) is thought to be involved in hereditary spastic paraplegias.

Immunogen

KLH-conjugated linear peptide corresponding to human KIF5A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in mouse brain nuclear extract.

Western Blot Analysis: 1 µg/mL of this antibody detected KIF5A in 15 µg/mL of mouse brain nuclear extract.

Specificity

This antibody can recognize isoforms KIF5A, KIF5B, and KIF5C.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~117 kDa observed. Uncharacterized bands may appear at ~45 kDa and ~57 kDa in some lysates.